Cdks put Treslin on TopBP1
نویسندگان
چکیده
منابع مشابه
Cdks put Treslin on TopBP1
K umagai et al. describe how cy-clin-dependent kinases (Cdks) initiate DNA replication by promoting the interaction of two proteins conserved from yeast to mammals. In budding yeast, Cdks phosphorylate two replication origin proteins called Sld2 and Sld3, allowing them to bind a third protein called Dpb11. Together, these proteins recruit Cdc45, which then promotes the activation of the MCM hel...
متن کاملDNA Replication: Mammalian Treslin–TopBP1 Interaction Mirrors Yeast Sld3–Dpb11
There are many parallels between DNA replication in yeast and humans. Now, two recent studies extend this relationship by dissecting key conserved interactions necessary for initiation of the replisome.
متن کاملTreslin Collaborates with TopBP1 in Triggering the Initiation of DNA Replication
TopBP1 has important roles in both DNA replication and checkpoint regulation in vertebrates. We have identified a protein called Treslin that associates with TopBP1 in Xenopus egg extracts. Depletion of Treslin from egg extracts strongly inhibits chromosomal DNA replication. Binding of Treslin to chromatin in egg extracts occurs independently of TopBP1. However, loading of the initiator protein...
متن کاملRegulation of DNA Replication through Sld3-Dpb11 Interaction Is Conserved from Yeast to Humans
Cyclin-dependent kinases (CDKs) play crucial roles in promoting DNA replication and preventing rereplication in eukaryotic cells [1-4]. In budding yeast, CDKs promote DNA replication by phosphorylating two proteins, Sld2 and Sld3, which generates binding sites for pairs of BRCT repeats (breast cancer gene 1 [BRCA1] C terminal repeats) in the Dpb11 protein [5, 6]. The Sld3-Dpb11-Sld2 complex gen...
متن کاملDirect regulation of Treslin by cyclin-dependent kinase is essential for the onset of DNA replication
Treslin, a TopBP1-interacting protein, is necessary for deoxyribonucleic acid (DNA) replication in vertebrates. Association between Treslin and TopBP1 requires cyclin-dependent kinase (Cdk) activity in Xenopus laevis egg extracts. We investigated the mechanism and functional importance of Cdk for this interaction using both X. laevis egg extracts and human cells. We found that Treslin also asso...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2011
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.1936iti1